Animation of red blood cells (RBCs) moving inward and forward due to the action of optical forces, forming an effective waveguide of light. Credit: Light: Science & Applications, doi: 10.1038/s41377-019-0142-1 New photonic tools for medical imaging can be used to understand the nonlinear behavior of laser light in human blood for theranostic applications. When light enters biological fluids it is quickly scattered, however, some cell suspensions can induce nonlinear responses in laser beams to self-focus and enhance the penetration of light for biomedical applications as a quantifiable marker of disease. In a recent study now published in Light: Science and Applications, Rekha Gautam and her colleagues at the San Francisco State University and an international team of co-workers showed that a laser beam shining through red blood cell suspensions could become “self-trapped.” The process reduced light scattering to retain the power of the beam of laser light within the biological samples. The observed nonlinearity depended on osmotic conditions and the age of the samples. The scientists propose using the technique to diagnose sickle cell anemia or malaria; diseases which impact the size and shape of blood cells. Osmotic conditions play an important role in the properties of human red blood cells (RBCs) crucial during disease analysis. Numerous efforts in the past decade have focused on the study of the biomechanical properties of RBCs suspended in varying osmotic solutions. In the present work, Gautam et al. determined the self-trapping and scattering-resistant nonlinear propagation of a laser beam through three different osmotic solutions/conditions. The results showed that the strength of the optical nonlinearity increased with osmotic pressure on the cells. Interestingly, in aged blood samples with lysed cells the nonlinear behavior was notably different due to the presence of free hemoglobin. To explain the experimental observations, Gautam et al. used a theoretical model with an optical force-mediated nonlocal nonlinearity. The present work on light self-guiding through scattered soft biological matter can introduce new photonic tools for noninvasive biomedical imaging and medical diagnosis. The scientists obtained blood samples from anonymous donors for the experiments. In the first set of experiments, they used a linearly polarized continuous wave (CW) laser beam with a wavelength of 532 nm. They focused the light into a 3 cm long glass cuvette filled with RBC suspensions in diverse osmotic conditions, as previously described. They monitored the linear and nonlinear outputs from the sample using a CCD camera and power detector, and measured the beam diameters using the Beamview program. The beam first diffracted normally at a low power of 10 mW and experienced strong scattering thereafter due to random distribution of non-spherically shaped RBCs. Gautam et al. then measured normalized laser transmission (output/input power) as a function of the input beam power. In hypotonic solutions, they noted the RBCs were in a “swollen” state where the effective refractive index of the cells decreased as the water-to-Hb ratio increased. In contrast, in the hypertonic solution, the scientists observed that RBCs shrunk, and their effective index increased due to reduced water-to-Hb ratio. In a third isotonic solution, the cells exhibited a “normal” state, in which the RBCs showed intermediate behavior. When the experiments were performed using the same blood samples two weeks later, the scientists observed notably different outcomes in which the nonlinear focus dramatically enhanced for the hypertonic solution. New shapes of laser beam ‘sneak’ through opaque media More information: Rekha Gautam et al. Optical force-induced nonlinearity and self-guiding of light in human red blood cell suspensions, Light: Science & Applications (2019). DOI: 10.1038/s41377-019-0142-1 I. M. Vellekoop et al. Exploiting disorder for perfect focusing, Nature Photonics (2010). DOI: 10.1038/nphoton.2010.3 Roarke Horstmeyer et al. Guidestar-assisted wavefront-shaping methods for focusing light into biological tissue, Nature Photonics (2015). DOI: 10.1038/nphoton.2015.140Roadmap on structured light. Journal of Optics. iopscience.iop.org/article/10. … 978/19/1/013001/meta UPPER PANEL: Normalized transmission and output beam size as a function of input power. a Measurement of the normalized transmission and b output beam size change in fresh RBC suspensions of different buffer solutions. The cyan (triangle) curve depicts the results obtained from the PBS background solution without RBCs as a reference, which indicates no appreciable self-action of the beam in the buffer solution itself. The blue (circle), red (square), and green (diamond) curves show the data obtained from RBC suspensions in hypertonic, isotonic, and hypotonic solutions, respectively, where the error ranges in (b) are indicated by the shaded regions. c Corresponding results from the same blood sample but after the RBCs have been stored in a refrigerator for two weeks, where the nonlinear focusing is dramatically enhanced in the hypotonic solutions. LOWER: Optical gradient forces on RBCs under different osmotic conditions examined by optical tweezers. a–c Snapshots of RBC movement towards a 960-nm laser beam (position marked by a dashed green circle) in isotonic, hypotonic, and hypertonic solutions, respectively, as observed under a microscope. The red arrows illustrate the directional cell movement. d–f Power spectrum analyses showing the trap stiffness κx of a single RBC from the three suspensions in accordance with (a–c), where the vertical dashed lines mark the corner frequency fc. The inset in (f) illustrates a single RBC that moves into the trap under the action of the gradient force. Credit: Light: Science & Applications, doi: 10.1038/s41377-019-0142-1. Explore further Self-trapping light through human RBC suspensions under different osmotic conditions. a–c Illustrations of the beam dynamics in (a) isotonic, (b) hypotonic, and (c) hypertonic suspensions. d Side-view image of a self-trapped beam. e–g Observed output intensity patterns at a low power, which show the linear diffraction and strong scattering of the laser beam. i–k Corresponding patterns at a high power, which show the beam localization due to nonlinear self-trapping. h, l 3D plots of the intensity patterns corresponding to (g, k), respectively. Credit: Light: Science & Applications, doi: 10.1038/s41377-019-0142-1. , Light: Science & Applications Nonlinear optical response of lysed RBCs (free hemoglobin) in water. a Output beam size as a function of input power through the Hb solutions for four different concentrations. The RBC concentrations for the four curves (Hb1-Hb4) are 2.4, 5.1, 8.6, and 15.0 million cells per mL. Nonlinear self-focusing of the beam occurs at ~100 mW for high concentrations of Hb, but it subsequently expands into thermal defocusing rings at high powers. b–e Typical output transverse intensity patterns taken for the self-trapped beam (b, d) and thermally expanded beam (c, e) for low (d, e) and high (b, c) concentrations. Credit: Light: Science & Applications, doi: 10.1038/s41377-019-0142-1 In this way, Gautam et al. studied nonlinear beam propagation in human RBCs suspended in three diverse buffer solutions. They found that RBCs exhibited a strong self-focusing nonlinearity that could be chemically controlled based on the buffer solution. They therefore propose tuning the optical nonlinearity via osmosis and increased osmotic pressure, outside the cells in fresh blood samples. When the samples aged, free hemoglobin from the lysed RBCs played an active role in the observed optical nonlinearity and enhanced the nonlinear response in hypotonic conditions. Using direct video microscopy and optical tweezer measurements, the scientists showed that the beam trapping force was greatest for RBCs in the hypertonic conditions and weakest for hypotonic solutions. The scientists introduced a theoretical model to validate the observed experimental effects. The work will introduce a new perspective in the development of diagnostic tools as the results are very promising towards the development of laser treatment therapies for blood-related diseases. In a second set of experiments, the scientists used a home-built optical tweezer system to measure the optical gradient force on RBCs. Gautam et al. collected the forward-scattering light from the trapped cells with a condenser lens and subsequently focused onto a position sensitive detector (PSD). They calculated the stiffness and gradient force in the three separate solutions. To simplify the measurements, Gautam et al. treated hypotonic and hypertonic RBCs as disk shaped objects. They used a CCD camera to record cell movements from the three different solutions along with a microscope with two objectives, where the setup was driven using a 960 nm laser beam. The results illustrated the movement of cells against Brownian motion under the action of optical forces based on the conditions of the cell (shape, size) and their beam trapping capacity. Gautam et al. estimated the trapping force using the Langevin equation and informed that the force followed a trend of hypertonic > isotonic > hypotonic conditions.The scientists then developed a model to simulate nonlinear beam propagation in biological soft matter in order to understand the physics of optical force-mediated nonlinearity. They modelled time evolution of the particle concentration distribution using a diffusion-advection equation and considered the presence of a forward-scattering force to push the particles along the direction of beam propagation, alongside the optical gradient force. Gautam et al. calculated the change in beam size for the different gradient and scattering force parameters to simulate the nonlinear self-focusing effects under different buffer conditions. They recorded the changing size, volume and refractive indices of RBCs under diverse osmotic conditions that were accountable for the varying magnitude of optical forces that modified the optical nonlinearity. The simulated results were qualitatively consistent with the experimental observations. Pathophysiological conditions such as sickle cell anemia, malaria and sepsis are often closely related to the physical properties of RBCs, their shape and size. The fundamental features of varying refractive indices and cell shapes allow RBCs to react to changes in different osmotic environments making them ideal candidates to study scattering light. In the present work, Gautam et al. showed nonlinear self-trapping of light across a centimeter distance of propagation by scattering RBC suspensions. When they increased the power of the laser beam, they showed the beam dramatically self-focus within all three osmotic conditions – much like optical spatial solitons (nonlinear self-trapped wave packets). The optical forces that change with cell density and morphology can provide noninvasive tools to sort diverse cells, according to a specific stage of a given disease. © 2019 Science X Network Journal information: Nature Photonics Human RBCs are disc-shaped malleable cells that possess a spatially uniform refractive index as they lack nuclei unlike most organelles, and show distinctive deformability for passage through veins and microcapillaries. The shape change can be prompted by modifying the osmolarity of the surrounding liquid buffer to use RBCs as tunable optofluidic microlenses. The optical properties of RBCs are important for in vitro and in vivo disease diagnostics in which the refractive index of the RBC is determined by hemoglobin (Hb)—the largest part of the erythrocyte dry content by weight. As a result, if the cell volume decreased due to varying osmotic conditions, the refractive index increased. Simulations of the optical force-induced nonlinear beam dynamics in RBC-like suspensions. a–c Beam size (FWHM) change as a function of the gradient and scattering forces obtained via numerical simulations using a 350-mW input power and neglecting random scattering effects, where one observes the change in beam size when either the gradient or the scattering force is “turned off”. d, f Side-view of the beam propagation and e, g corresponding output transverse intensity patterns after propagating through an RBC-like random scattering medium at low (d, e) and high (f, g) beam power. The beam side-views and output intensity patterns are normalized with respect to their respective maximal input powers. Credit: Light: Science & Applications, doi: 10.1038/s41377-019-0142-1. Citation: Optical force-induced self-guiding light in human red blood cell suspensions (2019, March 21) retrieved 18 August 2019 from https://phys.org/news/2019-03-optical-force-induced-self-guiding-human-red.html This document is subject to copyright. Apart from any fair dealing for the purpose of private study or research, no part may be reproduced without the written permission. The content is provided for information purposes only.